EO Biological Indicators Suppliers & Manufacturers in Jayashankar Bhupalpally

Self-Contained Biological Indicators for EO/EtO Sterilization

Medi Gases offers reliable and easy-to-use Self-Contained Biological Indicators (SCBIs) to monitor the effectiveness of your EO/EtO (Ethylene Oxide) sterilization processes. Our biological indicators contain bacterial spores on a paper carrier, packaged within a thermoplastic culture tube, offering a simple yet accurate method to verify the sterility of your products.

Procedure:

• Placement: Simply place the biological indicator inside your products or product packages before sterilization.
• Sterilization: Place the “inoculated package” in the EO/EtO sterilizer and process according to your standard procedures.
• Activation: After sterilization, remove the biological indicator and “activate” it by crushing the soft plastic culture tube. This action breaks the glass ampoule, allowing the medium to mix with the spores.
• Incubation: Place the activated biological indicator in an incubator for:
  • Steam sterilization: 24 hours at 35–39°C
  • EO/EtO sterilization: 48 hours at 35–39°C
• Result: If any spores survive the sterilization process, the culture medium will turn yellow, indicating the presence of viable organisms. If no growth is detected, the sterilization process has been successful.

Key Features

• Easy-to-Use: Provides a simple visual result in just 24–48 hours without the need for laboratory transfers.
• Quick and Reliable: Monitors EO/EtO sterilization with accuracy, giving fast results that are easy to interpret.
• Non-invasive: No need for complicated handling or laboratory testing — the process can be done directly in the sterilization area.

Product Specifications

• Organism Used: Bacillus Atrophaeus (Subtilis var. Niger) 10E6
• Sterilization Process: EO Gas
• Incubation Time: 48 hours
• Incubation Temperature: 35 – 39°C
• Reorder Number: SBI-E6